| F Kassie, EF Lhoste, A Bruneau, M Zsivkovits,
F Ferk, M Uhl, T Zidek, and S Knasmuller
Effect of intestinal microfloras from vegetarians and meat eaters on the genotoxicity of 2-amino-3-methylimidazo[4,5-f]quinoline, a carcinogenic heterocyclic amine.
J Chromatogr B Analyt Technol Biomed Life Sci, March 25, 2004; 802(1): 211-5.
Institute of Cancer Research, University of Vienna, Borschkegasse 8A A-1090 Vienna, Austria.
Aim of this study was to investigate the impact of intestinal microfloras
from vegetarians and non-vegetarians on the DNA-damaging activity of 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline
(IQ), a carcinogenic heterocyclic amine that is found in fried meats.
Floras from four vegetarians (Seventh Day Adventists [who tend not to
be vegan - ljf]) and from four individuals who consumed high amounts of
meats were collected and inoculated into germfree F344 rats. The rats
were kept on isocaloric diets that either contained animal derived protein
and fat (meat consumers group) or proteins and fat of plant origin (vegetarian
groups). IQ (90 mg/kg bw) was administered orally, after 4 h the extent
of DNA-damage in colon and liver cells was determined in single cell gel
electrophoresis assays. In all groups, the IQ induced DNA-migration was
in the liver substantially higher than in the colon. In animals
harbouring floras of vegetarians, the extent of damage was in both organs
significantly (69.2% in the liver, P<0.016 and 64.7%, P<0.042
in the colon, respectively) lower than in the meat consumer groups.
Our findings show that diet related differences in the microfloras have
a strong impact on the genotoxic effects of IQ and suggest that heterocyclic
amines are less genotoxic and carcinogenic in individuals that consume
mainly plant derived foods.
J Nutr. 2004 Feb;134(2):459-64.
Colonic bacterial flora: changing understandings in the molecular age.
Mai V, Morris JG Jr.
Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
The human intestinal microbiota is a complex bacterial consortium that is critical to normal health. The microflora is present at concentrations of 10(11)-10(12) cells/g of intestinal contents; the number of species present may exceed 500, although exact numbers remain to be defined, due in part to the fact that <30% of microorganisms are culturable with current microbiologic methods. Molecular tools based on 16S rDNA sequence similarities such as fluorescent in-situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), quantitative dot blot hybridization, restriction fragment length polymorphism (RFLP) and large scale 16S rDNA sequencing have helped to overcome limitations of conventional microbiological plating methods in studying the fecal microflora composition. However, these tools are just now beginning to be applied to understand the dynamics of this complex community, and its relationship to diet and human health. There is a need to understand both the limitations of the current data and the importance of moving forward with the best possible molecular and epidemiologic techniques as we deal with these critical questions.
Br J Nutr 2003 Apr;89(4):509-15
Protein-degradation products and bacterial enzyme activities in faeces of breast-fed and formula-fed infants.
Heavey PM, Savage SA, Parrett A, Cecchini C, Edwards CA, Rowland IR Northern Ireland Centre for Diet and Health, Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Northern Ireland, UK. email@example.com
The aim of the present study was to determine the effects of age and diet (breast milk, formula milk and weaning diet) on metabolic activities in faecal samples from infants aged 1 week to 1 year, and to compare these findings with activities found in samples from adults. Such activities can provide valuable information on functional changes in the microbiota that may have significance for the health of the host. Fresh faecal samples were collected from forty-four breast-fed infants (twenty-four males, twenty females) and thirteen formula-fed infants (three males, ten females) throughout the first year of life. The samples were analysed for protein-breakdown products, including the faecal concentrations of NH3, phenol and p-cresol, and faecal bacterial enzyme activities. There was wide individual variation in all variables measured; however, the values in infants were substantially lower then those found in adults. In pre-weaned infants, faecal NH3 concentration and beta-glucuronidase activity were the only endpoints that were significantly different in breast-fed and formula-fed infants (P<0.001 and P<0.05 respectively). This was not apparent after weaning. There was a significant difference between the breast-fed and formula-fed weaned groups and their pre-weaned counterparts only for NH3 (P<0.05). beta-Glucuronidase activity and phenol concentration were significantly (P<0.01) greater in weaned breast-fed infants compared with pre-weaned breast-fed infants. No differences were observed between pre-weaned and weaned formula-fed infants for any of the variables except for NH3 concentration. It can be concluded from the present study that there are significant differences in two faecal characteristics between breast- and formula-fed infants and that changes occur as the infants grow older and are weaned onto solid foods.
Am J Clin Nutr 2000 Dec;72(6):1488-94
Contribution of dietary protein to sulfide production in the large intestine: an in vitro and a controlled feeding study in humans.
Magee EA, Richardson CJ, Hughes R, Cummings JH. Dunn Clinical Nutrition Centre, Hills Road, Cambridge, United Kingdom. firstname.lastname@example.org
BACKGROUND: Hydrogen sulfide
is a luminally acting, bacterially derived cell poison that has been
implicated in ulcerative colitis. Sulfide generation in the colon is
probably driven by dietary components such as sulfur-containing amino
acids (SAAs) and inorganic sulfur (eg, sulfite).
Br J Nutr 2002 Sep;88 Suppl 1:S11-8
Intestinal flora during the first months of life: new perspectives.
Edwards CA, Parrett AM Department of Human Nutrition, Glasgow University, Yorkhill Hospitals, G3 8SJ, UK. email@example.com
Increasing awareness that the human intestinal flora is a major factor in health and disease has led to different strategies to manipulate the flora to promote health. The complex microflora of the adult is difficult to change in the long term. There is greater impact of diet on the infant microflora. Manipulation of the flora particularly with probiotics has shown promising results in the prevention and treatment of diarrhoea and allergy. Before attempting to change the flora of the infant population in general, a greater understanding of the gut bacterial colonisation process is required. The critical stages of gut colonisation are after birth and during weaning. [when toxic cultural diets are introduced - ljf] Lactic acid bacteria dominate the flora of the breast-fed infant. The formula-fed infant has a more diverse flora. [due to the introduction of foods not in our evolutionalry histor - ljf] The faeces of the breast-fed infant contain mainly acetic and lactic acid whereas the formula fed-infant has mainly acetic and propionic acid. Butyric acid is not a significant component in either group. The formula-fed infant also has higher faecal ammonia and other potentially harmful bacterial products. [infant 'formula' generally has animal proteins which produce toxic byproducts, just as in the meat/dairy-eating afult - ljf] The composition of the microflora diversifies shortly before and particularly after weaning. The flora of the formula-fed infant develops more quickly than that of the breast-fed infant. Before embarking on any strategy to change the flora, the following questions should be considered: Should we retain a breast-fed style flora with limited ability to ferment complex carbohydrates? Can pro- and prebiotics achieve a flora with adult characteristics but with more lactic acid bacteria in weaned infants? Are there any health risks associated with such manipulations of the flora?
Am J Clin Nutr 1999 Sep;70(3 Suppl):594S-600S
Rheumatoid arthritis treated with vegetarian diets.
Kjeldsen-Kragh J Department of Immunology and Transfusion Medicine, Ullevaal University Hospital, Oslo, Norway. firstname.lastname@example.org
The notion that dietary factors may influence rheumatoid arthritis (RA) has been a part of the folklore of the disease, but scientific support for this has been sparse. In a controlled, single-blind trial we tested the effect of fasting for 7-10 d, then consuming an individually adjusted, gluten-free, vegan diet for 3.5 mo, and then consuming an individually adjusted lactovegetarian diet for 9 mo on patients with RA. For all clinical variables and most laboratory variables measured, the 27 patients in the fasting and vegetarian diet groups improved significantly compared with the 26 patients in the control group who followed their usual omnivorous diet throughout the study period. One year after the patients completed the trial, they were reexamined. Compared with baseline, the improvements measured were significantly greater in the vegetarians who previously benefited from the diet (diet responders) than in diet nonresponders and omnivores. The beneficial effect could not be explained by patients' psychologic characteristics, antibody activity against food antigens, or changes in concentrations of prostaglandin and leukotriene precursors. However, the fecal flora differed significantly between samples collected at time points at which there was substantial clinical improvement and time points at which there were no or only minor improvements. In summary, the results show that some patients with RA can benefit from a fasting period followed by a vegetarian diet. Thus, dietary treatment may be a valuable adjunct to the ordinary therapeutic armamentarium for RA.
Acta Physiol Hung 1999;86(3-4):171-80
Vegan diet in physiological health promotion.
Hanninen O, Rauma AL, Kaartinen K, Nenonen M. Department of Physiology, University of Kuopio, Finland.
We have performed a number of studies including dietary interventions and cross-sectional studies on subjects consuming uncooked vegan food called living food (LF) and clarified the changes in several parameters related to health risk factors. LF consists of germinated seeds, cereals, sprouts, vegetables, fruits, berries and nuts. Some items are fermented and contain a lot of lactobacilli. The diet is rich in fiber. It has very little sodium, and it contains no cholesterol. Food items like berries and wheat grass juice are rich in antioxidants such as carotenoids and flavonoids. The subjects eating living food show increased levels of carotenoids and vitamins C and E and lowered cholesterol concentration in their sera. Urinary excretion of sodium is only a fraction of the omnivorous controls. Also urinary output of phenol and p-cresol is lowered as are several fecal enzyme levels which are considered harmful. The rheumatoid arthritis patients eating the LF diet reported amelioration of their pain, swelling of joints and morning stiffness which all got worse after finishing LF diet. The composite indices of objective measures showed also improvement of the rheumatoid arthritis patients during the intervention. The fibromyalgic subjects eating LF lost weight compared to their omnivorous controls. The results on their joint stiffness and pain (visual analogue scale), on their quality of sleep, on health assessment questionnaire and on general health questionnaire all improved. It appears that the adoption of vegan diet exemplified by the living food leads to a lessening of several health risk factors to cardiovascular diseases and cancer. Rheumatoid patients subjectively benefited from the vegan diet which was also seen in serum parameters and fecal analyses.
Appl Environ Microbiol 1992 Nov;58(11):3660-6
Comment in: Appl Environ Microbiol. 1993 Aug;59(8):2763-4.
An uncooked vegan diet shifts the profile of human fecal microflora: computerized analysis of direct stool sample gas-liquid chromatography profiles of bacterial cellular fatty acids.
Peltonen R, Ling WH, Hanninen O, Eerola E. Department of Medical Microbiology, Turku University, Finland.
The effect of an uncooked extreme vegan diet on fecal microflora was studied by direct stool sample gas-liquid chromatography (GLC) of bacterial cellular fatty acids and by quantitative bacterial culture by using classical microbiological techniques of isolation, identification, and enumeration of different bacterial species. Eighteen volunteers were divided randomly into two groups. The test group received an uncooked vegan diet for 1 month and a conventional diet of mixed Western type for the other month of the study. The control group consumed a conventional diet throughout the study period. Stool samples were collected. Bacterial cellular fatty acids were extracted directly from the stool samples and measured by GLC. Computerized analysis of the resulting fatty acid profiles was performed. Such a profile represents all bacterial cellular fatty acids in a sample and thus reflects its microflora and can be used to detect changes, differences, or similarities of bacterial flora between individual samples or sample groups. GLC profiles changed significantly in the test group after the induction and discontinuation of the vegan diet but not in the control group at any time, whereas quantitative bacterial culture did not detect any significant change in fecal bacteriology in either of the groups. The results suggest that an uncooked extreme vegan diet alters the fecal bacterial flora significantly when it is measured by direct stool sample GLC of bacterial fatty acids.
J Nutr 1975 Jul;105(7):878-84
Effects of high risk and low risk diets for colon carcinogenesis on fecal microflora and steroids in man.
Reddy BS, Weisburger JH, Wynder EL.
We investigated the effects of a high meat mixed Western diet and a nonmeat diet, representing the dietary pattern of high and low risk areas for colon cancer, respectively, on fecal microflora dn on bile acid and neutral sterol patterns in man. The total anaerobic microflora as well as the count of Bacteroides, Bifidobacterium, Peptococcus, and anaerobic Lactobacillus were significantly higher during the period of consumption of a high meat mixed Western diet comparted with the nonmeat-diet consumption period. The difference in total fecal bile acid excretion was not significant between the two dietary periods. Fecal excretion of microbially modified bile acids and neutral sterols was decreased when subjects eating a high meat diet transferred to a nonmeat diet. These results support the fact that diet plays a modifying role on the composition of intestinal microflora, bile acids, and neutral sterols.